Transperineal versus transrectal prostate biopsy for predicting the final laterality of prostate cancer: are they reliable enough to select patients for focal therapy? Results from a multicenter international study

Objectives: To compare the concordance of prostate cancer (PCa) laterality between the extended transperineal (TP) or transrectal (TR) prostate biopsy (BP) and radical prostatectomy (RP) specimens. To identify predictors of laterality agreement between BP and RP. Materials and Methods: Data from 533 consecutive patients with PCa (278 TP and 255 TR-diagnosed) treated with RP were analyzed. A 12-core technique was used for both TP and TR biopsies. Additional cores were obtained when necessary. Results: Overall, the percentage of agreement of PCa laterality between BP and RP was 60% (K = 0.27, p < 0.001). However, the RP confirmation of unilaterality at BP was obtained in just 33% of the cases. Considering the concordance on bilaterality as the “target” of our analysis, the sensitivity and specificity were 54.3% and 98.2%, respectively, with TP and 47.5% and 92.5%, respectively with TR. Focusing on patients with unilaterality at biopsy, none of the evaluated preoperative variables (biopsy technique, age, total positive biopsy cores, PSA, prostate volume, Gleason score on biopsy) were able to predict RP bilaterality in the multivariate analyses. Conclusions: Most of the patients with unilateral involvement at BP harbored bilateral PCa after RP. TR and TP biopsy showed no difference in their capacity to predict the concordance of tumor laterality at RP. None of the preoperative evaluated variables can predict the tumor laterality at RP. Using BP unilaterality to include patients in focal therapy (FT) protocols may hinder the oncologic efficacy of FT. .

Morphological Study of the Liver in the Teleost Oreochromis niloticus / Estudio Morfológico del Hígado en el Teleósteo Oreochromis niloticus

Liver samples of Oreochromis niloticus cultivated in floating net cages were fixed for histological and ultrastructural studies with the objective of describing the hepatic parenchymal structure and the intrahepatic exocrine pancreatic tissue. Anatomically, the liver showed only two hepatic lobes. Histological analysis demonstrated that the hepatocytes were spread out as anastomotic cords, arranged in two cellular layers and surrounded by sinusoids. The intrahepatic exocrine pancreatic tissue exhibited an acinar arrangement and was diffused in the hepatic parenchyma. Structural analysis showed that the hepatocytes had a rounded nucleus and a rough endoplasmic reticulum with a parallel disposition to the nuclear membrane. The exocrine pancreatic cells showed secretion granules at the apical portion and the rough endoplasmic reticulum was concentrically distributed.

Con el objetivo de describir la estructura del parénquima hepático y del páncreas exocrino intrahepático del Oreochromis niloticus, fueron fijados para estudios histológicos y ultraestructurales fragmentos de hígado de peces cultivados en jaulas flotantes. Se evidenciaron sólo 2 lóbubos hepáticos. El análisis histológico demostró que los hepatocitos se encontraban organizados en forma de cordones anastomosados, dispuestos en dos capas celulares y cercados por sinusoides. El tejido pancreático exocrino intrahepático se encontró difuso en el parénquima hepático y se destacó por su organización acinar. El análisis ultraestructural demostró que los hepatocitos presentaban núcleos redondos y el retículo endoplasmático rugoso estaba dispuesto paralelamente a la membrana nuclear. Las células pancreáticas exocrinas presentaban gránulos de secreción localizados en la porción apical y el retículo endoplasmático rugoso estaba organizado de manera concéntrica.

Histochemical study in the epididymis of the dog canis familiaris

Para la determinación de glucosaminoglicanos en el epidídimo del perro fueron usadas reacciones histoquímicas. En los tres segmentos del epitelio ductal la determinación de mucosustancias neutras reveló reacciones medias. No obstante, la presencia de gránulos PAS positivos en el epitelio, indicarían una probable secreción glucoproteica de las células principales. Las determinaciones histoquímicas confirman las interpretaciones previas

Estrutura da prostata da cobaia (Cavia porcellus, L): reexame da histologia do complexo secretorio. / Structure of the prostate of guinea pigs (Cavia porcellus, L): histological reexamination of the secretory complex

No presente trabalho e descrita a morfologia da prostata da cobaia ao nivel do microscopio optico. Observou-se que esta glandula possui dois lobos: dorsal maior e ventral menor, localizada nos paquimeros correpondentes. A estrutura glandular e mucosa havendo predominio da configuracao tubular e alveolar no lobo dorsal. O epitelio secretor esta formado por celulas basais e principais no lobo ventral. Aparentemente, nao se observam celulas basais no lobo dorsal, no qual se obervaram tambem linfocitos intra-epiteliais. A secrecao celular e clara, hialina e tem como caracteristicas: eosinofilia e PAS-positividade

Vascularizacao arterial pelvica em pequenos mamiferos, domesticos e de laboratorio. Estudo comparativo. / Arterial vascularization of the pelvis of domestic and laboratory small mammalians.Comparative study

A vascularizacao arterial da pelve em gato, coelho e roedores de laboratorio no que concerne as origem, ramescencia e distribuicao dos vasos pelvicos (inclusive dos ramos terminais da aorta), foi estudada. Nestes estudos foi descrito, com especial enfase, o comportamento arterial dos ramos viscerais das arterias iliacas internas (aa. umbilical e urogenital), concernente a vascularizacao dos orgaos pelvicos nestas especies. Estas informacoes sao agregadas e relatadas no presente trabalho e todos os dados sao comparativamente discutidos com outras informacoes da literatura especializada